The aim of the project is to optimize the state-of-art of OMICS methods for targeted acquisition of microorganisms useful in xenobiotics decontamination (primarily persistent pesticides). Particular aim is to obtain microbial isolates useful in decontamination of the persistent chloracetanilide pesticides including their metabolites, which are legislativelly regulated in the Czech Republic. Among applicable results belong also a certified technology for pesticides and their metabolites removal from water and validated methods for the detection of chloracetanilide pesticides and their metabolites in soil matrix. The general aim is to contribute to the national action plan, i.e. to reduce the risks related to pesticides use.